Fig. 11.

Hyperproliferation in the Aey69 mutant eye. a) Antibodies labeling diverse retinal cell types were used to characterize the retina at P7. The wild-type images clearly indicate that at P7 there is a stratified retina with distinct cell types: Calbindin-positive horizontal and amacrine cells, PKCα-positive bipolar cells, OTX2-positive photoreceptors and bipolar cells, GFAP-positive Müller cells, and BRN3-positive retinal ganglion cells. In the Aey69, these cell types were present covering the entire ‘empty lens area’ of the mutant eye. The bars indicate 50 μm; n = 3 for each embryonic stage; L, lens; R, retina; ON, optic nerve. b) KI67 immunostaining was used to characterize proliferation in the developing eye of wild types and mutants. The results indicate differences in the distribution of KI67 between the wild-type and mutant eyes. At E15.5, in the wild-type retina KI67 positive cell population seems to be restricted to the future outer neuroblastic layer (marked by arrows). However, in the mutant the arrows indicate that the region occupied by the Ki67-stained cells is comparatively larger. The bars indicate 100 μm; n = 3 for each embryonic stage. L, lens; R, retina; ON, optic nerve.