Table 1.
Description of A. fumigatus isolates in the study including minimum inhibitory concentrations.
| Isolation date | Strain | Genotype | Number of non-synonymous single nucleotide polymorphisms different | Minimum inhibitory concentration (MIC; mg/L) |
|||
|---|---|---|---|---|---|---|---|
| Itraconazole | Voriconazole | Posaconazole | Amphotericin B | ||||
| 22/11/11 | V130-15 | WT cyp51A | 1 | 1 | 0.25 | 0.5 | |
| V130-15svf1213* | 213* in svf1 | 1 | 1 | 0.25 | 0.5 | ||
| V130-15unc167* | 167* in AFUA_7G01960 | 4 | 2 | 0.25 | 0.5 | ||
| 9/12/13 | V157-39cyp51AG54R | G54R in cyp51A | 21 different from V130-15 35 different from V157-62 |
>16 | 1 | >16 | 0.5 |
| V157-39cyp51AWT | WT cyp51A | 0.5 | 1 | >16 | 0.5 | ||
| 9/12/13 | V157-62 | M220R in cyp51A; 213* in svf1; 167* in AFUA_7G01960 | 29 different from V130-15 35 different from V157-39 |
>16 | 8 | >16 | 0.25 |
Bold indicates a MIC exceeding the EUCAST clinical resistance breakpoint; which are defined as itraconazole > 2 mg/L, voriconazole > 2 mg/L, posaconazole > 0.25 mg/L and amphotericin B > 2 mg/L.