Figure 4.

miR-101 expression was regulated by MANCR in GC cells. The one-way ANOVA and Tukey post hoc test revealed that expression levels of (A) MANCR and (B) miR-101 were significantly increased 24 h following transfection compared with the C and NC groups. (C) MANCR overexpression resulted in miR-101 downregulation in GC cells compared with the C and NC groups. (D) miR-101 overexpression did not alter MANCR expression compared with the C and NC groups. (E) MANCR siRNA mediated the upregulation of miR-101 compared with the C and NC groups. *P<0.05. miR, microRNA; MANCR, mitotically associated long-non coding RNA; GC, gastric cancer; C, control; NC, negative control; si, small interfering.