Figure 3. Differential effect of inflammatory signals from pancreatic tumors on IL12a^GFP expression in B cell subsets.

(A) Frequency of GFP⁺ B cell subsets isolated from spleens of KPC orthotopic tumor-bearing and non-tumor bearing (naïve) Il12a^GFP mice measured by flow cytometry; n=6, *=p<0.05, ns=p>0.05.

(B) Mean fluorescent intensity (MFI) of GFP in GFP⁺ B cell subsets isolated from spleens and tumors of KPC orthotopic tumor-bearing and non-tumor bearing (naïve) Il12a^GFP measured by flow cytometry mice; n=6, *=p<0.05, ns=p>0.05.

(C) Frequency of GFP⁺ B cell subsets isolated from orthotopic KPC pancreatic tumors of Il12a^GFP mice measured by flow cytometry; n=6.

(D) Proportions of CD19⁺GFP⁺ cell populations isolated from Il12a^GFP orthotopic KPC pancreatic tumors; n=6.