Table 1
| Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| anti-CD11c | Biolegend | N/A |
| anti-CD19 | Biolegend | N/A |
| anti-CD1d | BD Biosciences | N/A |
| anti-CD21 | Biolegend | N/A |
| anti-CD23 | Biolegend | N/A |
| anti-CD4 | Biolegend | N/A |
| anti-CD40 | Biolegend | N/A |
| anti-CD45 | Biolegend | N/A |
| anti-CD5 | Biolegend | N/A |
| anti-CD8 | Biolegend | N/A |
| anti-CD93 | BD Biosciences | N/A |
| anti-IgD | eBioscience | N/A |
| anti-IgM | Jackson Immune Research | N/A |
| anti-p35 | eBioscience | N/A |
| CD11b | BD Biosciences | N/A |
| CD3 | Biolegend | N/A |
| True Stain FCX | Biolegend | N/A |
| Bacterial Strains | ||
| Stellar competent cells | Takara Biosciences | N/A |
| Chemical | ||
| 2-mercaptoethanol | Thermo Scientific | N/A |
| Brefeldin A | Biolegend | N/A |
| buprenorphine | ||
| CO2 | ||
| CpG ODN1826 | Invivogen | N/A |
| cytofix/cytoperm buffer | BD | N/A |
| DMEM | Gibco | N/A |
| EDTA | Thermo Scientific | N/A |
| FCS | Corning | N/A |
| Gentamicin | Thermo Scientific | N/A |
| IC fixation buffer | eBioscience | N/A |
| Ionomycin | Cell Signalling | N/A |
| ketamine | ||
| L-glutamine | Thermo Scientific | N/A |
| LPS | Sigma | N/A |
| OpiPrep | Sigma | N/A |
| PBS | Thermo Scientific | N/A |
| Pen/strep | Thermo Scientific | N/A |
| Perfecta SYBR Green supermix | Quanta Biosciences | N/A |
| PMA | Sigma | N/A |
| R848 | Invivogen | N/A |
| RBC lysis solution | eBioscience | N/A |
| sodium Azide | sigma | N/A |
| Tris | Fisher Scientific | N/A |
| Xylazine | ||
| Critical Commercial Assay | ||
| B cell isolation kit | Stem Cell Technologies | N/A |
| Maxima First Strand cDNA synthesis kit | Thermo Scientific | N/A |
| Qiagen Hi Speed maxiprep kit | Qiagen | N/A |
| RNeasy minikit | Qiagen | N/A |
| Experimental models | ||
| mice | Charles River | C57Bl6/J |
| Oligonucleotides | ||
| b-actin fwd | Invitrogen | 5’-GGCTGTATTCCCCTCCATCG–3’ |
| b-actin rev | Invitrogen | 5’-CCAGTTGGTAACAATGCCATGT-3’ |
| emGFP fwd | Invitrogen | 5’-GACCACCTTGACCTACGGCG-3’ |
| emGFP rev | Invitrogen | 5’ –CCCTCGAACTTCACCTCGGC – 3’ |
| Il12a fwd | Invitrogen | 5’-CATCGATGAGCTGATGCAGT-3’ |
| Il12a rev | Invitrogen | 5’-CAGATAGCCCATCACCCTGT-3’ |
| ProteinPeptide | ||
| Collagenase IV | Worthington | N/A |
| DNAseI | Worthington | N/A |
| Flap endonuclease | NEB | N/A |
| Hyaluronidase | Worthington | N/A |
| Matrigel | Corning | N/A |
| Phusion polymerase | NEB | N/A |
| Soy Bean Trypsin Inhibitor | Sigma | N/A |
| Zombie NIR | Biolegend | N/A |
| Zombie Violet | Biolegend | N/A |
| Software | ||
| BD FACS DIVA | BD | N/A |
| FlowJo | TreeStar | N/A |
| Prism 8 | Graph Pad | N/A |