Table 2.
Complete list of vectors and plasmids used in the study.
| Plasmid | Protein (s) | Function |
|---|---|---|
| pCAGGS | None | Expression vector |
| pCAGGS-gpK8.1-gB-gL-gH −HR2 | KSHV glycoproteins, K8.1, gB, gH and gL without NDV F protein heptad repeat 2 domain |
VLP production |
| pCAGGS-gpK8.1-gB-gL-gH+HR2 | KSHV glycoproteins, K8.1, gB, gH, gL and NDV F protein heptad repeat 2 domain |
VLP production |
| pCAGGS-NDV NP | NDV nucleocapsid protein | VLP production |
| pCAGGS-NDV M | NDV matrix protein | VLP production |
| pCAGGS-NP LANA1 | Truncated KSHV latent associated nuclear antigen | Immunoblot |
| pCAGGS-LANA1 | KSHV wild type latent associated nuclear antigen | Immunoblot |
| pCAGGS-gpK8.1 | KSHV glycoprotein K8.1 | Stable cell production/ protein production |
| pCAGGS-gB | KSHV glycoprotein gB | Soluble protein production |
| pCAGGS-gH/gL | KSHV glycoproteins gH and gL | Soluble protein production |
| pCMVi-SV40ori-Fc-His | None | Expression vector |
| pCMVi-SV40ori-gpK8.1-Fc-6xHis | KSHV glycoprotein K8.1 | Soluble protein production |
| pCMVi-SV40ori-gB-Fc-6xHis | KSHV glycoprotein gB | Soluble protein production |
| pCMVi-SV40ori-gH/gL- Fc-6xHis | KSHV glycoproteins gH and gL | Soluble protein production |