Table 1:
Sampling and storage of Massospora-infected cicadas and their asymptomatic cicada counterparts for morphological, molecular, metabolomics, and genomic studies. Sample origin refers to the authors/lab who collected specimens. Numbers before the slash denote conidial isolates whereas those after denote resting spore isolates. Bracketed numbers denote isolates for which sequences were deposited in NCBI Genbank.
| Cicada host/population | Massospora sp. | Year collected/processed | Sample origin | Sample storage | Tissues sampled | Morphology | Phylogenetics | Global Metabolomics | Targeted Metabolomics | Genomics |
|---|---|---|---|---|---|---|---|---|---|---|
| Magicicada septendecula Brood I (WV, USA) | Mas.cicadina | 1978/2017 | JRC/CS | In 95.0% ethanol @4 °C | Fungal spores (FS) | 1/0 | 1/0 | – | – | – |
| Magicicada spp. Brood II (NY & CT, USA) | Mas.cicadina | 1979/2017 | JRC/CS, RAH | Dried and pinned, RT | FS | 3/0 | 1/0 | – | – | – |
| Magicicada septendecula Brood IV or XIX (MO, USA) | Mas.cicadina | 1998/2017 | JRC/CS | Dried and pinned, RT | FS | 1/0 | – | – | – | – |
| Magicicada spp. Brood V (OH & WV, USA) | Mas.cicadina | 2016/2017–2018 | MTK, NA, WJD | Freshly collected to −20 °C | FS | 5/0 | 3/0 [1] | 5/0 | 20/0 | 1/0 |
| Magicicada septendecim Brood VI (NC, USA) | Mas.cicadina | 2017/2017–2018 | TG | Freshly collected to −20 °C | FS | 0/8 | 0/3 | 0/4 | 0/4 | – |
| Magicicada septendecim Brood VIII (PA, USA) | Mas.cicadina | 2002/2017–2018 | JRC/CS | In 95.0% ethanol @4 °C | FS, concentrated ethanol(CE) | 1/0 | – | – | 0/5 | – |
| Magicicada cassini Brood IX (NC, USA) | Mas.cicadina | 2003/2018 | JRC/CS | In 95.0% ethanol @4 °C | CE | – | – | – | 0/1 | – |
| Magicicada cassini Brood X (OH, USA) | Mas.cicadina | 2004/2017 | GK | Dried and pinned, RT | FS | 1/0 | 1/0 | – | – | – |
| Magicicada septendecula Brood XIV (OH, USA) | Mas.cicadina | 2008/2017 | GK | Dried and pinned, RT | FS | 1/0 | 1/0 | – | – | – |
| Magicicada septendecula Brood XIX (IL, USA) | Mas.cicadina | 1998/2017 | JRC/CS | Dried and pinned, RT | FS | 0/3 | 1/0 | – | – | – |
| Magicicada tredecim Brood XXII (MS, USA) | Mas.cicadina | 2001/2018 | JRC/CS | In 95.0% ethanol @4 °C | CE | – | – | – | 3/0 | – |
| Magicicada spp. Brood XXIII (IL & IN, USA) | Mas.cicadina | 1989, 1998, 2002, 2015/2017–2018 | JRC/CS, GK | In 95.0% ethanol @4 °C | FS, CE | 2/0 | 1/0 [1] | – | 0/4 | – |
| Okanagana rimosa (MI) | Mas. levispora | 1998/2017 | JRC/CS | In 95.0% ethanol @4 °C | CE | 8/1 | 3/0 [1] | – | 7/1 | – |
| Platypedia putnami (NM) | Mas. platypediae | 2017/2017–2018 | MTK | Freshly collected to −20 °C | FS | 14/1 | 3/0 [1] | 5/0 | 11/0 | 1/0 |
| Magicicada spp. Brood V | None/healthy | 2016/2017–2018 | MTK | Freshly collected to −20 °C | Healthy abdomen (HA) | – | – | 1 | 1 | – |
| Okanagana rimosa (MI) | None/healthy | 1998/2017–2018 | JRC/CS | In 95.0% ethanol @4 °C | HA | – | – | – | 1 | – |
| Platypedia putnami (NM) | None/healthy | 2017/2017–2018 | MTK | Freshly collected to −20 °C | HA | – | – | 1 | 1 | – |
| Magicicada sp. Brood VII | None/healthy | 2018/2018 | MTK | Freshly collected to −80 °C | HA | – | – | – | 10 | – |
| Platypedia putnami (CA) | None/healthy | 2018/2018 | MTK | Freshly collected to −80 °C | HA | – | – | – | 10 | – |
| Total | 50 | 18 | 16 | 79 | 2 |