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. 2019 Nov 15;9:1163. doi: 10.3389/fonc.2019.01163

Figure 3.

Figure 3

miR-184 enhances apoptosis and G2/M phase arrest of retinoblastoma (RB) cells in response to etoposide (ETO) treatment. (A) Y79 cells with different transfection were treated with ETO (0.25 μM) for 48 h. Cellular apoptosis was detected by flow cytometry. Statistical analysis of the Annexin V+PI+-positive cell ratio in Y79 cells (B) and WERI cells (C). Same as in (A), apoptosis-related mRNA expressions in Y79 cells with different transfection alone (D) or additionally treated with ETO (E) were detected by qRT-PCR. (F) Western blot analysis of the expression of apoptosis-related proteins in Y79 cells with indicated treatment same as in (A). (G) Cell cycle distribution of Y79 cells with treatment same as in (A) was detected by flow cytometry. Statistical analysis of cell cycle phase ratio in Y79 cells (H) and WERI cells (I). (J) γH2AX foci in Y79 cells with indicated treatment same as in (A) was detected by immunofluorescence. Statistical analysis of γH2AX foci in Y79 cells, which were counted in at least 100 randomly selected cells per sample. Data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.0001 vs. negative control group.