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. 2019 Nov 15;9:1163. doi: 10.3389/fonc.2019.01163

Figure 4.

Figure 4

SLC7A5 is a direct target of miR-184. (A) Bioinformatics algorithms (TargetScan, Starbase, and miRTarBase) predicted the target of miR-184. Venn diagram found that TNPO2 and SLC7A5 are two shared targets of miR-184. The expressions of SLC7A5 and TNPO2 in Y79 cells with different transfection were detected by Western blot (B) and qRT-PCR (C). (D) The three putative miR-184 binding sites in 3′UTR of SLC7A5. (E) The wild-type (WT) or mutant (Mut) reporter constructs was cotransfected with negative control or miR-184 mimic into Y79 cells, and the dual luciferase activity was determined at 48 h after transfection. (F) The WT or Mut reporter constructs were cotransfected with negative control or miR-184 inhibitor into Y79 cells, and the dual luciferase activity was determined at 48 h after transfection. (G) SLC7A5 expression in normal retina and Rb tissues was detected by immunohistochemistry. (H) Spearman correlation analysis showed that SLC7A5 mRNA level is negatively associated with miR-184 in 15 RB tissues. Data were presented as mean ± SD of three independent experiments. ***P < 0.0001 vs. negative control group.