Fig. 2. Mtb-infected MHV68^POS mice have a transient increase in the frequency of IFNγ-producing CD4⁺ cells.

C57BL/6 mice were infected with MHV68 and Mtb H37Rv as depicted in Fig. 1A. On post-Mtb infection days 17 and 38, we determined the frequency of MLN CD4⁺ and CD8⁺ cells capable of producing IFNγ following PMA/ionomycin stimulation. Results are representative of three independent experiments; significance values are indicated above the data, as determined using Student's t-test.