Table 3.

Basal characterization of vehicle and E2-treated male mice

Characteristic	Vehicle	E2
Body weight (g)	31.6 ± 1.2	30.6 ± 0.6
Body fat (%)	17.6 ± 2.3	8.1 ± 1.4**
Lean mass (g)	20.5 ± 0.5	22.3 ± 0.6*
Plasma testosterone (ng ml−1)	1.25 ± 0.3	1.0 ± 0.3
Fasting insulin (μU ml−1)	14.8 ± 3.0	6.9 ± 1.7*
Clamped insulin (μU ml−1)	46.4 ± 9.4	42.1 ± 4.4
Glucose (mg dl−1)	166.1 ± 14.6	150.4 ± 7.8
Energy expenditure (kcal kg−1 h−1)	14.8 ± 0.6	17.0 ± 0.5*
RER $\left({\dot{V}}_{{\text{O}}_2}/{\dot{V}}_{\text{C}{\text{O}}_2}\right)$	0.78 ± 0.005	0.79 ± 0.005
Caloric intake (kcal kg−1 h−1)	16.2 ± 1.4	20.3 ± 1.1*
Volume drunk (ml kg−1 h−1)	1.0 ± 0.1	1.5 ± 0.1*
Activity (counts h−1)	205 ± 23	173 ± 34

Data are means ± SEM of n = 8–10 mice per group.

^*P < 0.05,

^**P < 0.01 compared with vehicle-treated mice.