Figure 6. Pulmonary +/+ macrophage transplantation restores Df-induced IL-12p40 production and neutrophil infiltration during sensitization of P2ry6^fl/fl/Cre/+ mice.

(A) BM macrophages from +/+ or P2ry6^fl/fl/Cre/+ mice were administered (i.t.) into +/+ and P2ry6^fl/fl/Cre/+ mice: +/+ macrophages into +/+ mice (+/+→+/+, n = 11); P2ry6^fl/fl/Cre/+→+/+ (n = 4); P2ry6^fl/fl/Cre/+→P2ry6^fl/fl/Cre/+ (n = 7); and +/+→P2ry6^fl/fl/Cre/+ (n = 7). (B) P2ry6 transcript in BAL fluid cells of pulmonary macrophage transplantation–treated (PMT-treated) +/+ and P2ry6^fl/fl/Cre/+ mice on day 2 (17 days after transplantation). ^##P < 0.01 by parametric t test. (C) BAL fluid IL-12p40 from PMT-treated +/+ and P2ry6^fl/fl/Cre/+ mice on day 2. (D) Diff-Quick–stained BAL fluid cells from the indicated mice. Red arrows indicate neutrophils. Scale bar: 50 µm. (E) Percentages of neutrophils in BAL fluid from PMT-treated +/+ and P2ry6^fl/fl/Cre/+ mice. (F) MPO levels in BAL fluid of PMT-treated +/+ and P2ry6^fl/fl/Cre/+ mice on day 2. (G) BAL cell Mpo transcript levels of PMT-treated mice on day 2. Values are mean ± SEM. Data are from 2 experiments. *P < 0.05, **P < 0.01 vs. +/+→+/+ mice; ^#P < 0.05, ^###P < 0.001 vs. P2ry6^fl/fl/Cre/+→P2ry6^fl/fl/Cre/+ mice by 1-way ANOVA followed by Tukey’s multiple-comparisons test (C–G).