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. 2019 Nov 4;129(12):5357–5373. doi: 10.1172/JCI125212

Figure 6. HLA-B27 mediates the upregulation of the RARB/TNAP axis in AS MSCs.

Figure 6

(A) ARS staining of mineralization in AS MSCs transduced with shHLA-B or shCtrl under osteogenic induction with quantification (B). (C) Immunoblot analyses showing the expression of RARB and TNAP in AS MSCs expressing shHLA-B or shCtrl at day 7 under osteogenic induction. (D) Immunoblot showing HLA-B27 expressions of control MSCs transduced with pLAS2w or pLAS2w-HLA-B27. (E) ARS staining of control MSCs transduced with control lentiviral vector (pLAS2w) or a vector expressing HLA-B27 (pLAS2w-HLA-B27) with quantification (F). (G) Immunoblot analyses showing RARB and TNAP expressions in control MSCs transduced with pLAS2w or pLAS2w-HLA-B27. All experiments done in the AS patient group and the control group are from AS MSCs (A1, A2, and A3) and control MSCs (C1, C2, and C3), respectively, with at least 2–3 experimental repeats. Data are the mean ± SEM. ***P < 0.001; ****P < 0.0001 by 2-tailed Student’s t test (2 groups) or 1-way ANOVA, followed by Tukey’s HSD test. Representative images from AS (A1) MSCs are shown in C. Scale bars: 200 μm (A and E).