Figure 1. Murine Slc30a10 deficiency recapitulates human disease.

Eight-week-old Slc30a10^+/+ (+/+), Slc30a10^+/KO (+/KO), and Slc30a10^KO/KO (KO/KO) mice were characterized for (A) tissue Slc30a10/Hprt1 RNA ratios, normalized to levels in Slc30a10^+/+ females; (B) tissue weights as a percentage of body weight; (C) body weight; and (D) tissue Mn levels. (E) MRIs (sagittal views). For A–D, P values were calculated by 1-way ANOVA with Tukey’s multiple comparisons test. Data are presented as individual values and represent the mean ± SEM. Outliers (not shown) were identified by the ROUT method. Removal of outliers in A did not alter the identification of comparisons with a P value below 0.05. No outliers were identified in C or D. Removal of the outlier for male KO/KO liver (4.180%) in B decreased the P value versus +/KO from P > 0.05 to P < 0.0001. For all panels, n = 5–9 replicates/group, except for female Slc30a10^+/KO mice (n = 3–4). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. F, female; M, male.