Figure 11. Slc30a10 in both hepatocytes and enterocytes contributes to the regulation of Mn levels.

Slc30a10^lox/lox with or without Alb and/or Vil underwent ⁵⁴Mn/fluorescein injection via the portal vein with (+) or without (–) prior HPD ligation (Lig). Samples were collected after 1 hour. Data for females and males were pooled. Data are presented as individual values and represent the mean ± SEM. Outliers (not shown) were identified by ROUT. To compare groups with and without ligation or specific genotypes with lox/lox mice that underwent the same surgical treatment, 2-tailed P values were calculated by unpaired t test and are indicated above the brackets at the top of the panels: *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, for comparisons between mice of the same genotype with and without ligation; ^##P < 0.01 and ^###P < 0.001, for comparisons between the indicated mouse genotype and Slc30a10^lox/lox mice that underwent the same surgical treatment (ligation or no ligation). The values in parentheses represent P values for select groups. Removal of the outlier in A (0.060 in female Slc30a10^lox/lox Vil with ligation) changed the P value from P > 0.05 to P < 0.01. No outliers were identified in B–I. n = 6–11 replicates/group.