Figure 3. Tissue metal levels in 8-week-old WT mice raised on a control or high-Mn diet.

C57BL/6NJ mice weaned onto a control Mn (10 ppm) or high-Mn (2400 ppm) diet were characterized at 8 weeks of age for blood (A, C, E, and G) and tissue (B, D, F, and H) levels of Mn (A and B), iron (Fe) (C and D), copper (Cu) (E and F), and zinc (Zn) (G and H). Data are presented as individual values and represent the mean ± SEM. Outliers (not shown) were identified by ROUT. P values were calculated by unpaired, 2-tailed t test. Removal of the outliers in A and B did not alter the identification of comparisons with P values below 0.05. No outliers were identified in C–H. n = 5 replicates/group, except for females on a 10 ppm diet (n = 4–5). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.