Figure 4. Hepatobiliary Mn excretion is increased in WT mice raised on a high-Mn diet.

C57BL/6NJ mice weaned onto a control Mn (10 ppm) or high-Mn (2400 ppm) diet were characterized at 8 weeks of age. (A–D) ⁵⁴Mn levels in feces (A), urine (B), tissues/compartments (C–E), and bile (F) after ⁵⁴Mn injection and overnight housing in metabolic cages, presented as a percentage of total ⁵⁴Mn levels. (G and H) Total biliary Mn (G) and copper (H) levels. For A–H, data are presented as individual values and represent the mean ± SEM. Two-tailed P values were calculated by unpaired t test. Outliers (not shown) were identified by ROUT. No outliers were identified in A–F or H. Removal of the outliers in G did not alter the identification of comparisons with P values below 0 .05. (I) Bile flow rates following bile duct ligation and gallbladder cannulation in female (left) and male (right) mice. Each data point represents the mean ± SEM. The values shown in I indicate the average slope of the line ± SEM for each group followed by the P value for comparison of line slopes by linear regression. n = 5 replicates/group except for females on a control Mn diet (n = 4–5). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.