Figure 5. Slc30a10 is essential for systemic and hepatobiliary Mn excretion.

Eight-week-old Slc30a10^+/+ and Slc30a10^KO/KO mice were characterized for (A and B) fecal (A) and urinary (B) ⁵⁴Mn levels after retro-orbital ⁵⁴Mn injection and overnight housing in metabolic cages (data are presented as a percentage of total ⁵⁴Mn); (C and D) biliary Mn levels normalized to liver weight (C) and Mn content (D) (in D, biliary Mn levels from WT mice on a control or high-Mn diet are included for reference); and (E) biliary copper levels. (F–H) Mice underwent bile duct ligation, gallbladder cannulation, ⁵⁴Mn/fluorescein injection into the portal vein, and bile collection for 2 hours. (F) Blood and liver ⁵⁴Mn levels. (G and H) Bile volume (G) and biliary ⁵⁴Mn levels (H) versus time for female (left) and male (right) mice. Each data point represents the mean ± SEM. Values shown in G and H indicate the average slope of the line ± SEM for each group followed by a P value for comparison of line slopes by linear regression. In A–F, data are presented as individual values and represent the mean ± SEM. Outliers (not shown) were identified by ROUT. Two-tailed P values were calculated by unpaired t test. No outliers were identified in A–C, E, or F. Removal of the outlier in D did not alter the identification of comparisons with a P value below 0.05. For all panels, n = 5 replicates/group, except for females on a high-Mn diet (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.