Figure 6. Slc30a10 is localized to the canalicular hepatocyte membrane.

(A) RNA levels of transporters and cell type–specific markers relative to Hprt1 levels: albumin (hepatocytes), Tek (sinusoidal endothelial cells), Pdgfrb (stellate cells), and Adgre1 (Kupffer cells). For each gene, the cell type with higher expression was set to 1, with the other groups normalized to that value. (B) Tissue Mn levels in 8-week-old Slc30a10^+/+, Slc30a10^KO/GFP, and Slc30a10^GFP/GFP mice. (C) Fluorescence images of 2-month-old Slc30a10^+/+ and Slc30a10^GFP/GFP frozen liver sections. DAPI (blue); GFP (green). Original magnification, ×20; scale bars: 200 μm; original magnification, ×50 (enlarged inset). (D) Immunofluorescence images of frozen liver sections from 2-month-old Slc30a10^GFP/GFP mice. DAPI (blue); anti-MDR1 (α-MDR1) (red). Original magnification, ×20 and ×50 (enlarged inset); scale bars: 200 μm. In A and B, data are presented as individual values and represent the mean ± SEM. In A, 2-tailed P values were calculated by unpaired t test. n = 5 replicates/group. In B, removal of the outliers identified by the ROUT method (0.962, 0.931 for female Slc30a10^+/+ brain) resulted in a change in P value versus Slc30a10^GFP/KO from P > 0.05 to P < 0.01. P values were calculated by 1-way ANOVA with Tukey’s multiple comparisons test. n = 5–10 replicates/group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.