Figure 8. Hepatic Slc30a10 is essential for hepatobiliary Mn excretion.

Four-month-old Slc30a10^lox/lox and Slc30a10^lox/lox Alb mice were analyzed for fecal (A), urinary (B), and liver and bile (C) ⁵⁴Mn levels after ⁵⁴Mn injection, overnight housing in metabolic cages, gallbladder cannulation, and 1-hour bile collection. Levels are expressed as a percentage of total ⁵⁴Mn levels. (D) Total biliary Mn and copper levels, normalized to liver mass. (E and F) Another set of female (left) and male (right) mice underwent bile duct ligation, gallbladder cannulation, ⁵⁴Mn/fluorescein injection via the portal vein, and 1-hour bile collection. (E) Biliary volumes. (F) Bile ⁵⁴Mn levels. In A–D, data are presented as individual values and represent the mean ± SEM. Outliers (data not shown) were identified by ROUT. Two-tailed P values were calculated by unpaired t test. No outliers were detected in A–D. In E, each data point represent the mean ± SEM. Values shown in F indicate the average slope of the line ± SEM for each group followed by a P value for comparison of the line slopes by linear regression. For all panels, n = 4–7 replicates/group. *P < 0.05, **P < 0.01, and ***P < 0.001.