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. 2019 Oct 22;129(12):5092–5107. doi: 10.1172/JCI122767

Figure 1. Postnatal EC-specific KO of Casp-8 results in impaired angiogenesis.

Figure 1

(A) Schematic showing tamoxifen administration in pups. (B) Representative images of whole-mount P6 retinas stained with IsoB4 (ECs) in Casp-8WT and Casp-8ECKO mice. Dashed black lines highlight the total retina area. (C and D) Quantification of vessel area (C; n = 22 WT, n = 21 ECKO) and retina vessel outgrowth (D; n = 20 WT, n = 20 ECKO). (E) Representative higher magnifications of the retina stained with IsoB4. (F) Quantification of number of branches (n = 18 WT, n = 16 ECKO). (G) Representative images of the retina angiogenic front stained with IsoB4. Black arrows point to EC sprouts. (H) Quantification of the number of sprouts per front length showing reduced number of sprouts in Casp-8ECKO retinas (n = 16 WT, n = 12 ECKO). For C, D, F, and H, data are shown as mean ± SEM from 4 independent litters. *P < 0.05; ***P < 0.001, 2-tailed unpaired Student’s t test. Scale bars: 100 μm (B); 50 μm (E); 20 μm (G).