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. 2019 Nov 11;129(12):5600–5614. doi: 10.1172/JCI129388

Figure 2. Arp2/3 inhibition affects CTL killing.

Figure 2

(A) Killing capacity of OT-I CTLs treated with the inactive control compound CK689 or the Arp2/3 inhibitor CK666, expressed as a percentage of target cell lysis at the effector-to-target (E:T) ratios indicated (mean of 3 independent experiments; error bars indicate SEM). (B) Western blot of p-ERK1/2 and total ERK1/2 in nonstimulated (NS) cells or following stimulation with 1 μM OVA peptide or 50 nM PMA (for 15 minutes) in control versus treated cells (representative of 3 independent experiments). Numbers indicate the fold change (ratio) of p-ERK1 expression following stimulation and after normalization to total ERK1 expression. (C) Representative flow cytometry plot and quantitation (D) of LAMP1-PE (CD107a) uptake in OT-I CTLs in the absence (blue) or presence (red) of OVA-loaded EL4 (gated on CD8+ cells) after 3 hours following treatment with CK689 or CK666 (n = 3 independent experiments in duplicate).