TABLE 2.
Minimum inhibitory concentrations (μg/ml) of piperitenone epoxide (PEO) and antibiotics against clinical isolates of Escherichia coli and Staphylococcus aureus.
|
Minimum inhibitory concentration (μg/ml)∗ | |||||||||||||
| Strain | PEO | AMK | AMP | FEP | CAZ | CRO | LVX | LZD | MEM | MIN | PEN | TGC | VAN |
| E. coli 1 | 256 | 4 | 32 | 16 | 16 | 2 | 0.06 | nt | 2 | 2 | nt | 0.5 | nt |
| E. coli 2 | 512 | 4 | 32 | 4 | 32 | 4 | 0.12 | nt | 1 | 4 | nt | 0.5 | nt |
| E. coli 3 | 1024 | 1 | 8 | 4 | 32 | 4 | 2 | nt | 2 | 16 | nt | 0.25 | nt |
| E. coli 4 | 512 | 4 | 32 | 16 | 32 | 8 | 0.06 | nt | 2 | 2 | nt | 0.25 | nt |
| E. coli 5 | 512 | 4 | 32 | 32 | 16 | 64 | 2 | nt | 4 | 16 | nt | 0.12 | nt |
| E. coli 6 | 128 | 4 | 32 | 2 | 32 | 32 | 8 | nt | 2 | 16 | nt | 0.12 | nt |
| E. coli 7 | 128 | 4 | 32 | 8 | 16 | 64 | 8 | nt | 4 | 16 | nt | 0.12 | nt |
| E. coli 8 | 512 | 4 | 32 | 16 | 32 | 8 | 0.06 | nt | 2 | 2 | nt | 0.25 | nt |
| E. coli 9 | 1024 | 1 | 8 | 4 | 32 | 4 | 2 | nt | 2 | 16 | nt | 0.25 | nt |
| E. coli 10 | 512 | 4 | 8 | 2 | 32 | 8 | 0.06 | nt | 2 | 2 | nt | 0.25 | nt |
| E. coli 25922 | 512 | 1 | 4 | <0.5 | <8 | 0.05 | 0.06 | nt | 0.06 | 2 | nt | 0.25 | nt |
| S. aureus 1 | 256 | nt | 32 | nt | nt | 8 | 0.5 | 4 | 0.5 | 0.25 | 0.5 | 0.12 | 2 |
| S. aureus 2 | 64 | nt | 32 | nt | nt | 16 | 0.5 | 16 | 2 | 2 | 1 | 0.12 | 2 |
| S. aureus 3 | 128 | nt | 0.25 | nt | nt | 4 | 0.5 | 8 | 0.25 | 1 | 0.25 | 0.06 | 1 |
| S. aureus 4 | 128 | nt | 2 | nt | nt | 4 | 0.25 | 8 | 0.5 | 0.5 | 0.25 | 0.25 | 1 |
| S. aureus 5 | 64 | nt | 32 | nt | nt | 16 | 32 | 2 | 1 | 0.25 | 0.5 | 0.06 | 1 |
| S. aureus 6 | 128 | nt | 16 | nt | nt | 8 | 4 | 8 | 0.25 | 0.25 | 0.5 | 0.12 | 1 |
| S. aureus 7 | 128 | nt | 16 | nt | nt | 16 | 0.25 | 4 | 0.12 | 0.25 | 0.25 | 0.06 | 1 |
| S. aureus 8 | 256 | nt | 0.12 | nt | nt | 4 | 0.5 | 4 | 0.5 | 0.5 | 0.06 | 0.12 | 2 |
| S. aureus 9 | 128 | nt | 16 | nt | nt | 8 | 0.25 | 8 | 0.5 | 0.5 | 0.5 | 0.12 | 1 |
| S. aureus 10 | 64 | nt | 16 | nt | nt | 4 | 1 | 4 | 0.5 | 0.5 | 0.5 | 0.25 | 1 |
| S. aureus 11 | 256 | nt | 16 | nt | nt | 16 | 8 | 8 | 2 | 1 | 1 | 0.12 | 1 |
| S. aureus 12 | 128 | nt | 16 | nt | nt | 8 | 0.5 | 8 | 0.25 | 1 | 0.5 | 0.12 | 2 |
| S. aureus 13 | 128 | nt | 16 | nt | nt | 8 | 1 | 8 | 0.5 | 1 | 1 | 0.12 | 2 |
| S. aureus 14 | 64 | nt | 16 | nt | nt | 4 | 0.5 | 8 | 0.25 | 0.5 | 0.06 | 0.12 | 1 |
| S. aureus 15 | 256 | nt | 16 | nt | nt | 8 | 1 | 4 | 0.25 | 0.5 | 0.12 | 0.12 | 1 |
| S. aureus 16 | 256 | nt | 16 | nt | nt | 4 | 0.5 | 8 | 0.12 | 1 | 0.5 | 0.25 | 1 |
| S. aureus 17 | 256 | nt | 16 | nt | nt | 4 | 1 | 8 | 0.25 | 1 | 0.5 | 0.06 | 1 |
| S. aureus 18 | 32 | nt | 16 | nt | nt | 16 | 2 | 8 | 0.25 | 8 | 0.06 | 0.25 | 1 |
| S. aureus 19 | 256 | nt | 16 | nt | nt | 8 | 2 | 8 | 0.25 | 1 | 0.12 | 0.12 | 1 |
| S. aureus 20 | 64 | nt | 16 | nt | nt | 8 | 1 | 8 | 0.5 | 2 | 0.5 | 0.25 | 1 |
| S. aureus 21 | 32 | nt | 16 | nt | nt | 8 | 0.5 | 8 | 0.25 | 8 | 0.5 | 0.12 | 1 |
| S. aureus 22 | 128 | nt | 16 | nt | nt | 16 | 4 | 8 | 0.25 | 8 | 0.5 | 0.25 | 2 |
| S. aureus 23 | 32 | nt | 0.25 | nt | nt | 4 | 0.5 | 8 | 0.25 | 1 | 0.25 | 0.06 | 4 |
| S. aureus 24 | 32 | nt | 16 | nt | nt | 8 | 0.5 | 8 | 0.25 | 1 | 0.12 | 0.12 | 2 |
| S. aureus 25 | 1024 | nt | 16 | nt | nt | 16 | 0.12 | 4 | 0.12 | 0.25 | 0.12 | 0.06 | 2 |
| S. aureus 26 | 256 | nt | 0.12 | nt | nt | 4 | 0.5 | 16 | 0.25 | 0.5 | 0.06 | 0.12 | 2 |
| S. aureus 27 | 128 | nt | 16 | nt | nt | 8 | 1 | 4 | 0.25 | 0.5 | 0.5 | 0.12 | 1 |
| S. aureus 28 | 128 | nt | 16 | nt | nt | 4 | 1 | 8 | 0.25 | 0.5 | 0.12 | 0.25 | 1 |
| S. aureus 25923 | 64 | nt | 0.25 | nt | nt | 4 | 0.06 | 0.5 | 0.12 | 0.25 | 0.12 | 0.06 | 0.25 |
∗Mean values of three replicates, nt, not tested. Piperitenone epoxide (PEO), Amikacin (AMK), Ampicillin (AMP), Cefepime (FEP), Ceftazidime (CAZ), Ceftriaxone (CRO), Levofloxacin (LVX), Linezolid (LZD), Meropenem (MEM), Minocycline (MIN), Penicillin (PEN), Tigecycline (TGC), Vancomycin (VAN).