Table 1.

High throughput bulk sequencing of the T-cell receptor β chain for 5T4_p17-specific CD8⁺ T-cell clones isolated from healthy or kidney cancer donors

Donor	[5T4p17] stimulation (µg/mL)	Clone	TRB-CDR3 sequence	Frequency (%)	TRA/B single-cell sequencing
HD_A	10	1	CASSELPAGGTNEQFF	62.70	−
			CASSIYGETGTEAFF	35.20
		2	CASSELPAGGTNEQFF	97.94	+
	0.1	7	CASSFFSNTGELFF	99.20	−
		8	CASSFFSNTGELFF	98.17	−
		12	CASSFFSNTGELFF	98.52	−
		15	CASSFFSNTGELFF	97.94	+
HD_B	10	17	CASSQVSGYEQYF	95.48	−
		19	CASSQVSGYEQYF	99.54	+
	0.1	20	CASSLTSQGFQPQHF	65.25	+
			CASSPQGDNEQFF	34.06
		21	CASSPQGDNEQFF	99.02	+
		24	CASSPQGDNEQFF	95.96	−
HD_C	10	1	CASMDLAFKQYF	97.50	−
		3	CASMDLAFKQYF	98.41	+
		6	CASMDLAFKQYF	98.41	−
		11	CASMDLAFKQYF	98.74	−
	0.1	17	Null	63.66	+
			CASSYMGPEAFF	34.79
		18	Null	62.21	−
			CASSYMGPEAFF	36.81
		20	Null	62.80	−
			CASSYMGPEAFF	36.48
KCD_D	10	6	CASSFLTDTQYF	96.75	+

The bolded sequences were shared among clones from the same donor and peptide stimulation concentration

TRB T-cell receptor beta, CDR3 complementarity-determining region 3, TRA T-cell receptor alpha, HD healthy donor, KCD kidney cancer donor