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. 2019 Nov 25;9:17478. doi: 10.1038/s41598-019-53983-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RF-EME exposure does not induce γH2AX expression in the testis. Testis sections from untreated control animals (UT), as well as those of the sham and RF-EME exposure groups, were probed with anti-phospho-γH2AX antibodies (red) to detect DNA double strand breaks. (a) Representative images are depicted, with scale bar equating to 400 µm. A secondary antibody only control is also included. Corresponding DAPI (blue) stained images, illustrating tubule morphology are included as insets included in the upper right corner of each panel. (b) Analysis of pixel intensity was performed on the germ cell population within the seminiferous tubules in order to quantify γH2AX expression levels across treatments. Graphical data are presented as mean + SEM (n = 3 mice/treatment group, with 8–25 tubules being analyzed for each testis).