Table 1.
Number of replicates used for each assay.
| Assay/measurement | Untreated control |
1 week | 3 weeks | 5 weeks | |||
|---|---|---|---|---|---|---|---|
| Sham | EME | Sham | EME | Sham | EME | ||
| Body weight | NA | 20 | 20 | 14 | 14 | 8 | 8 |
| Testis histology | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Testis staining: γH2AX | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Testis staining: 4-hydroxynonenal | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| Sperm vitality | 5 | 6 | 6 | 5 | 6 | 8 | 8 |
| Sperm motility, progressive and rapid | 5 | 5 | 6 | 5 | 6 | 8 | 8 |
| Dihydroethidium staining | 4 | 6 | 9 | 5 | 8 | 8 | 8 |
| MitoSOX red staining | 6 | 6 | 12 | 5 | 11 | 11 | 11 |
| DNA fragmentation | 5 | 6 | 6 | 5 | 6 | 8 | 8 |
| Comet tail intensity | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
| 8OHdG | 3 | 4 | 4 | 4 | 5 | 5 | 5 |
| Tyrosine phosphorylation | 3 | NA | NA | NA | NA | 3 | 3 |
| Acrosome reaction | 3 | NA | NA | NA | NA | 3 | 3 |
| Sperm-zona pellucida adhesion | 3 | NA | NA | NA | NA | 3 | 3 |
| Fertilization | 7 | NA | NA | NA | NA | 3 | 5 |
| Blastocyst development | 3 | NA | NA | NA | NA | 3 | 3 |