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. 2019 Nov 25;10:5334. doi: 10.1038/s41467-019-13313-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — GRα is responsible for transcriptional activation of REG3A and REG1CP. a GRα silencing reduced REG3A expression. b, c GRα silencing reduced (b) whereas its overexpression increased (c) luciferase reporter activity of pGL3-basic based REG3 promoter constructs. d GRα bound to the 2325/-2509 fragment of REG3A promoter. The precipitates of an antibody against GRα from formaldehyde-cross-linked chromatin of cells were subjected to qPCR using primers directed to indicated fragments of REG3A promoter. e Silencing of REG1CP or FANCJ reduced the association between GRα and the 2325/-2509 fragment of REG3A promoter as shown using ChIP assays. f Silencing of REG1CP or FANCJ diminished the increase in luciferase reporter activity of pGL3-basic based REG3 promoter constructs (upper) and the upregulation of endogenous REG3A protein levels (lower) caused by overexpression of GRα. The number below each western blot lane represents the level of REG3A protein relative the level of GAPDH expression. g GRα bound to the −1094/−1244 fragment of REG1CP promoter as measured using ChIP assays. h Deletion of the −1094/−1244 fragment abolished luciferase reporter activity of pGL3-basic based REG1CP promoter constructs. i Overexpression of GRα increased luciferase reporter activity of pGL3-basic based REG1CP promoter constructs with the −1094/−1244 fragment. j GRα silencing decreased endogenous REG1CP expression. n = 3 independent experiments. Data are presented as Mean ± SEM (b, c, f (upper), h, i) or representatives (a, d, e, f (lower), g, j). Statistical significance was calculated using a two-tailed t-test.