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. 2019 Nov 19;10:809. doi: 10.3389/fendo.2019.00809

Figure 4.

Figure 4

Myotubes exposed to bone conditioned medium from cisplatin-treated mice display severe atrophy. Fully differentiated (5 days) C2C12 myotubes exposed to 20% bone conditioned medium (CM) from animals treated with V or C for up to 48 h. CM was generated by incubating the bones in medium for 48 h. Controls were exposed to either normal horse serum-containing differentiation medium (DM) or 20% αMEM-containing unconditioned medium (UCM). Myotubes were stained for Myosin Heavy Chain (MyHC, green) and myotube size was measured by using the ImageJ software. 250–300 myotubes were measured, n = 3. Scale bar: 100 μm. Images were recorded using a 10X magnification (insert: 20X). Data are expressed as means ± SEM. Significance of the differences: ***p < 0.001 vs. DM; $$p < 0.01 vs. UCM; &&p < 0.01 vs. V CM.