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. 2019 Nov 25;9:17462. doi: 10.1038/s41598-019-53846-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterial growth of MDRAB clinical isolates in the presence of H₂O₂. (a) Bacterial growth of E. coli (EC), ATCC 19606 (AB), and MDRAB clinical isolates cultured in LB broth were evaluated. (b) Growth is represented as a percentage of the growth of the corresponding untreated strain. Summarised results showing the growth of E. coli (EC), ATCC 19606 (AB), and MDRAB clinical isolates in the presence of 51 mM H₂O₂ in 6, 12, and 24-hour cultures. (c) Growth is represented as a percentage of the growth of EC, AB, and strain R1 in the presence of H₂O₂ at the concentrations indicated on the x axis, in 24-hour culture. (d) Growth is represented as a percentage of the growth of AB and strains R2 and R3 in the presence of H₂O₂ at the concentrations indicated on the x axis in 24-hour culture. (e) Growth is represented as a percentage of the growth of AB and strains R4 and R5 in the presence of H₂O₂ at the concentrations indicated on the x axis in 24-hour culture. Data are compiled from 2 independent experiments (n = 3 for each experiment), and are shown as mean ± SEM. (f) Pearson correlation coefficient was calculated between the percentage of growth in the presence of 51 mM H₂O₂ and the bacterial count in J774A.1 cells at 24 hours after phagocytosis (Pearson correlation coefficient r = 0.845, P = 0.010). Each symbol represents a clinical isolate, AB, or EC.