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. 2019 Nov 26;9:18009. doi: 10.1038/s41598-019-54634-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The obtained InvOLS values measured in 1 μm steps on the polystyrene substrate with the three FluidFM cantilevers. The curves represent a 5th grade polynomial fit on the datasets. (B) The spring constant measured in 1 μm steps in air with the three FluidFM cantilevers, as functions of the laser spot position.