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. 2019 Nov 25;9:17441. doi: 10.1038/s41598-019-53948-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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pXd-INV-D80A mutant complexes with hydroquinone as acceptor substrate. (A) Crystals soaked into fructose and HQ with a molecule of ethylene glycol trapped from the cryobuffer. (B) Crystals soaked into HQ, with two trapped ethylene glycol (EG) molecules and a molecule of Tris (TR) occupying the equivalent position of β-D-fructose in the ternary complexes. (C) Structural superimposition of the two complexes containing HQ (orange and violet) with the ternary complex with catechol (forest). Two positions are observed for HQ, one of them equivalent to catechol. Images created with software Pymol 1.7 (http://www.pymol.org/).