Table 2.
Plasmids used in this study.
| Plasmid | Description | References |
|---|---|---|
| pRSU1 | LEU2, ampR, PSUP35, SUP35 | Volkov et al., 2002 |
| pRSU1-sup35-M0 | LEU2, ampR, PSUP35, sup35-M0 | This study |
| pRSU2 | URA3, ampR, PSUP35, SUP35 | Volkov et al., 2002 |
| pRSU2-sup35-M0 | URA3, ampR, PSUP35, SUP35 | This study |
| pRS316CUP-NM-GFP | URA3, ampR, PCUP1, SUP35NM-GFP | Serio et al., 1999 |
| pRS316CUP-NM-M0-GFP | URA3, ampR, PCUP1, SUP35NM-M0-GFP | This study |
| pET-20b-SUP35NM-His6 | -, ampR, T7, SUP35-NM-His6 | Allen et al., 2005 |
| pET-20b-SUP35NM-M0-His6 | -, ampR, T7, SUP35-NM-M0-His6 | This study |
| pRS315CUP-NM-GFP | LEU2, ampR, PCUP1, SUP35NM-GFP | This study |
| pRS315CUP-NM-M0-GFP | LEU2, ampR, PCUP1, SUP35NM-M0-GFP | This study |
| pRS315 | LEU2, ampR | Sikorski and Hieter, 1989 |
| pRS315CG | LEU2, ampR, PCUP1, GFP | This study |
| pR16CUP-NM-yTagRFP-T | URA3, ampR, PCUP1, SUP35NM-yTagRFP-T | This study |
| pIM35 | URA3, ampR, PMET25, yTagRFP-T | Malcova et al., 2016 |
For each plasmid, the following characteristics are indicated: yeast selective marker, (“-” — the absence of a yeast selective marker), bacterial selective marker, promoter of the inserted gene, gene of interest. All yeast plasmids in the table are centromeric.