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. 2019 Nov 25;9:17449. doi: 10.1038/s41598-019-53868-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of predicted off-targets in NIH/3T3 and HeLa cell clones. Gene differential expression assay was performed on genes with over 20 reads, p value was less than 0.05. (a) Among the off-targets predicted for m-cas1, only Celf2, Teme147, Fkbp1a and Kdelr2 were significantly different among the clones. Celf2 and Teme147 were increased significantly in cas1-2 compared to control, while no difference was observed among other clones; Fkbp1a was downregulated in all clones, while Kdelr2 showed down or upregulation in clones compared to control. (b) Among the off-targets predicted for m-cas2, Slc7a11, Mrpl17 and Flna were differentially expressed. Slc7a11 and Mrpl17 were significant upregulated in cas1-2, while no changes detected in other clones. Flna was upregulated in cas2-1 and cas2-2, and no changes were found in other clones. (c) Among the off-targets predicted for h-cas1, H3F3B and JAK1 were upregulated in HeLa cell clones.