Table 1.
Predicted off-targets for gRNAs m-cas1.
| gene symbol | UCSC gene | sequence | mismatches | score |
|---|---|---|---|---|
| Vil1 | NM_009509 | GGGAAACTGGCTTCCTATGGGGG | 4MMs [1:6:11:15] | 0.1 |
| Mroh2a | NM_001281466 | AGGAAACTGGTGTTATCTGGAGG | 4MMs [6:12:14:17] | 0 |
| Tfcp2l1 | NM_023755 | AGGATGGTGGTTTCATAGGATGG | 4MMs [5:7:18:20] | 0.1 |
| Cfap221 | NM_001115074 | AAGATGCTGGTTTCACATGGAAG | 3MMs [2:5:16] | 0.5 |
| Atp2b4 | NM_213616 | TGGAAGCTGGGGTCATGTGGAGG | 4MMs [1:11:12:17] | 0.2 |
| mmu-mir-29b-2 | NR_029809 | TGGAAGCTGGTTTCACATGGTGG | 2MMs [1:16] | 2.3 |
| Cdkl1 | NM_183294 | AGGAAGCTGGGGTCATTTGCCAG | 4MMs [11:12:17:20] | 0.1 |
| Ngly1 | NM_021504 | AAGATGCTGGTTTTAAATGGGAG | 4MMs [2:5:14:16] | 0 |
| Cacna1d | NM_028981 | AGAAAGCTGGCCTCACATGGTGG | 4MMs [3:11:12:16] | 0.1 |
| A730017C20Rik | NR_033764 | AGAAACCTGGTTTCAGATGCCAG | 4MMs [3:6:16:20] | 0.1 |
| Rad9a | NM_011237 | AGGGAGTTAGTCTCATATGGGAG | 4MMs [4:7:9:12] | 0.3 |
| Celf2 | NM_001160293 | AGGAAAATGGTTTAATATTGGAG | 4MMs [6:7:14:19] | 0 |
| Fkbp1a | NM_008019 | AGGCAGCTAGTTTCAGAAGGTGG | 4MMs [4:9:16:18] | 0 |
| Kcnab2 | NM_001252655 | AGGAGGCTGGTCTCCTAGGGAGG | 4MMs [5:12:15:18] | 0 |
| Actr3b | NM_001004365 | AGCAAGTTGGTTTCACATGGTGG | 3MMs [3:7:16] | 0.4 |
| Kdelr2 | NM_025841 | AGGAGGCTGGCTTCAGCTGGGAG | 4MMs [5:11:16:17] | 0.1 |
| Vamp1 | NM_009496 | AGGAAGGTGGTGTCCTATGTTGG | 4MMs [7:12:15:20] | 0.1 |
| Tmem147 | NM_027215 | ATGAGGCTGGGTTCAAATGGGGG | 4MMs [2:5:11:16] | 0.1 |
| Bcl2a1d | NM_007536 | AGGAAGATGGCTTCATAAAGAAG | 4MMs [7:11:18:19] | 0 |
| Bcl2a1a | NM_009742 | AGGAAGATGGCTTCATAAAGAAG | 4MMs [7:11:18:19] | 0 |
| Bcl2a1b | NM_007534 | AGGAAGATGGCTTCATAAAGAAG | 4MMs [7:11:18:19] | 0 |
| Gm1720 | NR_046015 | AGGAAGCAGGTTTCACATATGGG | 4MMs [8:16:19:20] | 0 |