Table 2.

Predicted off-targets for gRNAs m-cas2.

gene symbol	UCSC gene	sequence	mismatches	score
Cfap221	NM_001115074	AAGATGCTGGTTTCACATGGAAG	3MMs [1:2:5]	2.4
mir-29b-1	NR_029532	AGGAAGCTGGTTTCATATGGTGG	2MMs [1:16]	2.3
Actr3b	NM_001004365	AGCAAGTTGGTTTCACATGGTGG	3MMs [1:3:7]	1.7
Vps53	NM_026664	TGGAAGCTGCCCTCACATGGCAG	3MMs [10:11:12]	0.6
Krtap9-5	NM_001085527	CAGCAGCTGGTTACACATGGTGG	4MMs [1:2:4:13]	0.6
Lrp1b	NM_053011	TGACATTTGGTTTCACATGGAGG	4MMs [3:4:6:7]	0.5
Cacna1d	NM_028981	AGAAAGCTGGCCTCACATGGTGG	4MMs [1:3:11:12]	0.4
Prr14l	NM_194340	TGGACACTGATTTCACATGAAAG	4MMs [5:6:10:20]	0.4
Vmn1r88	NM_001167537	TGGAAGATGGTGTCACTTGGAGG	3MMs [7:12:17]	0.4
Vmn1r86	NM_001167536	TGGAAGATGGTGTCACTTGGAAG	3MMs [7:12:17]	0.4
Slc7a1	NM_007513	CGGAAGCTGATCTCACCTGGTGG	4MMs [1:10:12:17]	0.3
Gm1720	NR_046015	AGGAAGCAGGTTTCACATATGGG	4MMs [1:8:19:20]	0.2
Cd5	NM_007650	GGGCAGGTGGTTTCACAGGGAGG	4MMs [1:4:7:18]	0.2
Zfp786	NM_177882	CGGAAGCTCGTGTCACATGTGGG	4MMs [1:9:12:20]	0.2
Clasp1	NM_177548	GGGAAGCTTGTATCACATTGGGG	4MMs [1:9:12:19]	0.2
Tpi1	NM_009415	TGGAAGGTGATTTCACTTGTCAG	4MMs [7:10:17:20]	0.2
St8sia1	NM_011374	TGGTACCTGGTTTCCCATGTGGG	4MMs [4:6:15:20]	0.1
Il21r	NM_021887	GGGGAGCTGGTTTGACTTGGAGG	4MMs [1:4:14:17]	0.1
Perm1	NM_172417	GGGCAGCTGGTTTCCCAAGGGAG	4MMs [1:4:15:18]	0.1
Efcab7	NM_145549	TGGAAGGTATTTTAACATGGAAG	4MMs [7:9:10:14]	0.1
Gnb3	NM_013530	TGGGAGGTGGTTTCAAATGTGAG	4MMs [4:7:16:20]	0.1
Npr3	NM_001039181	TGGAAGCTGGCTTCCCAGGGAAG	3MMs [11:15:18]	0.1
Tnpo3	NM_177296	GGGAAGTTGGTTTAACTTGGGGG	4MMs [1:7:14:17]	0.1
Mrpl1	NM_001039084	TTGATGCTGGTTTCAAAGGGAAG	4MMs [2:5:16:18]	0.1
Ush2a	NM_021408	TGGAAGCTTGCTTCTCGTGGGGG	4MMs [9:11:15:17]	0
Man2c1	NM_028636	TGGAAGCTGCATTCAGCTGGTGG	4MMs [10:11:16:17]	0
Flna	NM_010227	TGGAAGGTGGTGTCACAGGTAGG	4MMs [7:12:18:20]	0
Otop1	NM_172709	CGGAAGCTGGATTTACAGGGTGG	4MMs [1:11:14:18]	0
Atp2b4	NM_213616	TGGAAGCTGGGGTCATGTGGAGG	4MMs [11:12:16:17]	0
Lrrc2	NM_028838	TGGAACCTGGTTTCCCACTGTGG	4MMs [6:15:18:19]	0