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. 2019 Nov 26;10(12):891. doi: 10.1038/s41419-019-2128-6

Fig. 3. NDV infection induces the expression of pro-apoptotic transcription factor CHOP.

Fig. 3

a Induction of CHOP by NDV infection. HeLa cells were infected with NDV and harvested at indicated time points. The cell lysates were analyzed by western blot with antibodies against CHOP and NP. β-actin was detected as a loading control. b Nuclear translocation of CHOP during NDV infection. HeLa cells were infected with NDV or mock-infected for 16 h, subjected to immunostaining by using antibodies against CHOP and NP. The signal of CHOP (green) and viral protein NP (red) were observed under confocal microscope. c Knockdown of PKR or PERK reduces the phospho-eIF2α and expression of CHOP. HeLa cells were transfected with sic, siPERK, or siPKR, before infecting with NDV. Mock infection was set as control. At 20 h.p.i., cell samples were analyzed by western blot (upper panel) and quantitative real-time RT-PCR (low panel). The intensities of indicated protein bands were normalized to eIF2α or β-actin, respectively, and shown as fold change of siTarget:sic. Western blot and Immunofluorescence are representative of three independent experiments. CHOP mRNA level represents means ± SD of three independent determinations. *p < 0.05, ***p < 0.001.