Fig. 5.

JIL-1 and JASPer depletion in cells modulates the transcriptional output of genes, especially on the male X chromosome. a MA-plot showing mean log₂ fold-change of RNA-seq counts upon jasper RNAi versus control (upper panel, n = 4) and jil-1 RNAi versus controls (lower panel, n = 5) against mean RNA-seq counts for robustly detected genes at autosomes (left, chromosomes 2L, 2R, 3L, and 3R n = 6833) and X chromosome (right, n = 1441) in male S2 cells (left site). Statistically significant differentially expressed genes between RNAi and control conditions (fdr < 0.05) are marked in red and the number of significant genes is indicated on the plot. On the right, mean log₂ fold-change of RNA-seq counts upon jasper RNAi versus control (upper panel, n = 4) and jil-1 RNAi versus controls (lower panel, n = 4) against mean RNA-seq counts for autosomal genes (left, chromosomes 2L, 2R, 3L, and 3R n = 7144) and X chromosomal genes (right, n = 1509) in female Kc cells (left site). b Density plot showing mean log₂ fold-change of RNA-seq counts upon jasper RNAi versus controls (n = 4) and jil-1 RNAi versus controls (n = 5) at genes in male S2 cells, in left panel, as in a. X chromosomal genes (n = 1441) are marked with solid line and autosomal genes (chromosomes 2L, 2R, 3L, and 3R, n = 6833) with dashed line and jasper RNAi additionally in orange. Right panel, mean log₂ fold-change of RNA-seq counts upon jasper RNAi and jil-1 RNAi versus controls (n = 4 each) at genes in female Kc cells. X chromosomal genes (n = 1509) and autosomal genes (chromosomes 2L, 2R, 3L, and 3R, n = 7144).