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. 2019 Nov 19;13:517. doi: 10.3389/fncel.2019.00517

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Copyright © 2019 Hirrlinger, Marx, Besser, Sicker, Köhler, Hirrlinger, Wojcik, Eulenburg, Winkler and Hülsmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fate mapping of individual GGCN using COTRANS mice. In COTRANS mice, GGCN, which have a GABA and glycine cotransmitting phenotype will be irreversibly labeled by tdTomato expression. Examples of fate identification at p0 (A–D) and 2.5 months (E–H), by labeling tdTomato positive cells (A,E) for glycine (B,F) or GAD (C,G). (D,H) Overlay with tdTomato in purple, glycine in green and GAD in red. Examples of GGCN neurons are marked with a yellow arrow. Scale bar in (H) corresponds to 50 μm and applies to (A–H). Quantification of the cell populations at p0 (I) and 2.5 months (J). Pie charts show the mean values of n = 3 and 8 mice for p0 and 2.5 months, respectively. In total, 1133 and 1109 cells were counted for p0 and 2.5 months of age, respectively. 100% corresponds to all cells labeled by tdTomato expression. See Supplementary Figure 4 for the distribution of data.