TABLE 4.
Comparison of resting and behaviral breathing of VIACO mice (p127) (periods with maximal 10% vs. more than 50% behavioral breathing).
| Behavioral breathing | <10% | >50% | |||
| CTRL n = 13 | VIACO n = 6 | CTRL n = 12 | VIACO n = 6 | Test Parameter | |
| Respiratory rate [min–1] | 321.1 ± 54.2 | 289.3 ± 40.0 | 524.7 ± 32.8 | 515.1 ± 11.8 | PNT 0.944; PEVT < 0.05; FGT 2.057; PGT 0.161; FBhvl 220.895; PBhvl < 0.001; Fint 0.589; Pint 0.448 |
| §0.127 | # < 0.001 | §0.643, # < 0.001 | |||
| CV interval | 0.30 ± 0.12 | 0.38 ± 0.06 | 0.52 ± 0.07 | 0.65 ± 0.08 | PNT < 0.05; PEVT 0.665; FGT 9.892; PGT 0.004; FBhvl 61.024; PBhvl < 0.001; Fint 0.592; Pint 0.447 |
| §0.100 | # < 0.001 | §0.010, # < 0.001 | |||
| IrrScore Interval | 0.27 ± 0.08 | 0.33 ± 0.05 | 0.36 ± 0.06 | 0.43 ± 0.06 | PNT < 0.05; PEVT 0.918; FGT 7.645; PGT 0.009; FBhvl 15.981; PBhvl < 0.001; Fint 0.0563; Pint 0.814 |
| §0.081 | # < 0.001 | §0.043, # < 0.001 | |||
| Pause > 1s [min–1] | 0.00 ± 0.00 | 0.01 ± 0.03 | 0.00 ± 0.25 | 0.03 ± 0.06 | PNT < 0.05; PEVT 0.134; FGT 6.661; PGT 0.014; FBhvl 1.379; PBhvl 0.249; Fint 1.379; Pint 0.249 |
| §0.324 | #1.00 | §0.013, #0.162 | |||
| CV Amplitude | 0.23 ± 0.06 | 0.27 ± 0.05 | 0.21 ± 0.06 | 0.26 ± 0.11 | PNT < 0.05; PEVT 0.706; FGT 3.091; PGT 0.088; FBhvl 0.260; PBhvl 0.613; Fint 0.0621; Pint 0.805 |
| §n.t. | #n.t. | §n.t., #n.t. | |||
| IrrScore Amplitude | 0.22 ± 0.08 | 0.37 ± 0.39 | 0.25 ± 0.14 | 0.48 ± 0.61 | PNT < 0.05; PEVT = 0.402; FGT 3.403; PGT 0.074; FBhvl 0.437; PBhvl 0.513; Fint 0.131; Pint 0.720 |
| §n.t. | #n.t. | §n.t., #n.t. | |||
Time bins of respiration with less than 10% or more than 50% behavioral breathing (threshold 8 Hz) were analyzed separately. Data are shown as mean ± standard deviation (SD). Statistical analysis was performed with SigmaPlot 12.5 using two way ANOVA (General Linear Model; Degrees of Freedom: DFTotal 36) followed by Pairwise Multiple Comparison. PNT = P-value normality test; PEVT = P-value equal variance test; FBhvl = F-value for comparison of behavioral breathing, FGT = F-value for comparison of genotype; Fint = F-value for interaction; PBhvl = P-value for comparison of behavioral breathing; PGT = P-value for comparison of genotype; PInt = P-value for interaction. Pairwise Multiple Comparison were performed using the Holm-Sidak method. P-values for comparison between genotype per behavioral categories are marked as “§.” P-values for comparison between behavioral categories are marked as “#.” n: number of animals tested from each genotype and behavioral (Bhvl) group. Apneas (Pause > 1 s) are calculated per minute. n.t.: not tested.