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. 2019 Nov 19;10:2686. doi: 10.3389/fmicb.2019.02686

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Copyright © 2019 Zhou, Xu, Jiao, Yang, Chen, Callac, Liu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of in vitro PCR recombination events along the sequenced ITS DNA fragment. In the present study, base positions 1–32 are part of the 18S; positions 33–324 (type A), and 33–323 (types B and C) represent ITS1; positions 325–478 (type A) and 324–477 (types B and C) represent 5.8S; positions 479–694 (type A), 478–693 (type B), and 478–692 (type C) represent ITS2; and positions 695–731 (type A), 694–730 (type B), and 693–729 (type C) are part of the 26S. Because of the different lengths of types A, B, and C sequences, their aligned dataset was used to infer the regions where recombination occurred. Type A and C have the same 5.8S gene sequence, while type B has a different base at the position 366, adenine for type A and C, guanine for type B.