TABLE 1.
Mutation and recombination frequencies detected within each of the 11 PCR treatments.
| Treatment | Total | Identical sequences | Recombined sequences without mutated sites | Recombined sequences with mutated sites | Novel sequences without recombination | Total novel sequences | Accuracy rate (% ± SD)1 | |||||
| A | B | C | 1 | 2 | 3 | >3 | ||||||
| A (PCR) | 110 | 110 (100%) | – | – | – | – | – | – | – | – | – | 100 ± 0 a |
| B (PCR) | 109 | – | 109 (100%) | – | – | – | – | – | – | – | – | 100 ± 0 a |
| C (PCR) | 110 | – | – | 110 (100%) | – | – | – | – | – | – | – | 100 ± 0 a |
| A (Clone) | 653 | 633 (96.94%) | – | – | – | – | – | – | – | 20 (3.06%) | 20 (3.06%) | 96.94 ± 1.06 b |
| B (Clone) | 641 | – | 618 (96.41%) | – | – | – | – | – | – | 23 (3.59%) | 23 (3.59%) | 96.41 ± 1.47 b |
| C (Clone) | 648 | – | – | 624 (96.30%) | – | – | – | – | – | 24 (3.70%) | 24 (3.70%) | 96.30 ± 1.12 b |
| A+B | 621 | 243 (39.13%) | 190 (30.60%) | – | 101 (16.27%) | 43 (6.92%) | 20 (3.22%) | 3 (0.48%) | 8 (1.29%) | 13 (2.09%) | 188 (30.27%) | 69.51 ± 2.52 c |
| B+C | 630 | – | 204 (32.38%) | 221 (35.08%) | 108 (17.14%) | 50 (7.94%) | 23 (3.65%) | 3 (0.48%) | 5 (0.79%) | 16 (2.54%) | 205 (32.54%) | 67.33 ± 3.36 c |
| A+C | 635 | 218 (34.33%) | – | 231 (36.38%) | 105 (16.54%) | 44 (6.93%) | 16 (2.52%) | 1 (0.16%) | 7 (1.10%) | 13 (2.05%) | 186 (29.29%) | 69.91 ± 2.20 c |
| A+B+C | 636 | 150 (23.58%) | 122 (19.21%) | 136 (21.38%) | 112 (17.61%) | 56 (8.80%) | 28 (4.40%) | 4 (0.63%) | 11 (1.73%) | 17 (2.67%) | 228 (35.85%) | 64.65 ± 3.38 c |
| Original DNA | 636 | 169 (26.57%) | 131 (20.60%) | 138 (21.70%) | 103 (16.20%) | 47 (7.39%) | 15 (2.36%) | 2 (0.31%) | 11 (1.73%) | 20 (3.14%) | 198 (31.13%) | 68.07 ± 3.55 c |
| Total | 5429 | 1523 (28.05%) | 1374 (25.31%) | 1460 (26.89%) | 529 (16.75%)2 | 240 (7.60%)2 | 102 (3.23%)2 | 13 (0.41%)2 | 42 (1.33%)2 | 146 (3.69%)3 | 1072 (21.02%)3 | |
1Treatments with different letters a, b, and c indicate that the accuracy rates are statistically significantly different from each other. 2The results were calculated using the equation R = a/b, where a is the total recombined sequence of each item and b is the total sequence number of the treatments A+B, A+C, B+C, A+B+C and original DNA, i.e., 42/3158. 3The results were calculated using the equation R = x/y, where x is the sequence number of each item and y is the total sequence numbers of the treatments A (Clone), B (Clone), C (Clone), A+B, A+C, B+C, A+B+C and original DNA, i.e., 1072/5100.