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. 2019 Nov 19;13:1217. doi: 10.3389/fnins.2019.01217

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Copyright © 2019 Liu and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Immunofluorescent photomicrographs from the sections of the ARCN region stained for leptin receptor (OBR, in red), neuronal marker NueN (in green), and 4′,6-diamidino-2-phenylindole (DAPI, in blue) in a control and an HFD rat (X200). Amplified images are shown in the right panel. Arrow indicates the localization of OBR in the NeuN-labeled cell. (B) Immunofluorescent photomicrographs from the sections of the ARCN region stained for OBR (in red), glial marker glial fibrillary acidic protein (GFAP, in green), and DAPI (in blue) in a control and an HFD rat (X200). Amplified images are shown in the right panel. Arrow indicates the colocalization of OBR and GFAP. (C) Mean intensity of OBR staining signal in the ARCN of control and HFD rats. (D) Representative gel of OBR and mean protein expressions in the ARCN of control and HFD rats. ^∗P < 0.05 vs. control group.