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. 2019 Nov 19;7:286. doi: 10.3389/fcell.2019.00286

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Copyright © 2019 Qin, Tang, Li, Liu, Yang, Shi, Sun, Liu, Wang, Liang, Cong, Mo, Liu, Chen and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cell RNA-seq on TGF COCs showed affected process in ovulation. (A) Pathway enrichment revealed two significantly up-regulated pathways which potentially contributed to increased number of large antral follicles, and eight significantly down-regulated pathways that are involved in cumulus cell function and oocyte maturation. (B) Pathways in oocyte meiosis, progesterone-mediated oocyte maturation, ovarian steroidogenesis, and cell cycle, which are closely related to oocyte maturation and ovulation, showed DEGs enrichment of 22.4, 21.2, 25.5, and 22.6%, respectively. (C) qPCR validation of mRNA expression level of DEGs associated with the pathways in ovarian steroidogenesis (Cyp19, Star, Hsd17β) and AMPK (Prkaa1) pathway. (D) Quantification of mRNA level of genes related to oocyte meiosis indicated decreased expression of MPF, but unaffected expression of Npr2 during TGF follicular development. 90 d represents 90-day ovarian tissues. ^∗Stands for statistical significance (P < 0.05).