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. 2019 Nov 25;9:17461. doi: 10.1038/s41598-019-53972-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endothelialisation of silk vascular grafts. Data expressed as mean ± SEM, n = 4 animals/timepoint. The solid blue line represents the average of the bars presented (HFIP spun) and is compared to red dotted line which represents the average of water spun grafts (bars not shown). Statistical analysis comparing these averages was by two-way ANOVA, using Šídák multiple comparison test, with *p < 0.05, **p < 0.01, ***p < 0.001. P → D represents sections of the graft from proximal to distal. (A) Quantification of vWF coverage represented as a percentage of total luminal circumference. (B) Quantification of CD34 coverage represented as a percentage of total luminal circumference. (C) Representative images of cross sections at the mid graft of HS silk vascular grafts, white dotted lines indicate the graft wall. Top row: vWF stained in red and nuclei in blue. Bottom row: CD34 stained in red and nuclei in blue. Scale bar = 200 μm. Data for the WS group were modified from a previous study¹³.