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. 2019 Nov 25;9:17461. doi: 10.1038/s41598-019-53972-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage response to electrospun silk grafts. Data expressed as mean ± SEM, n = 4 animals/timepoint. Statistical analysis comparing these averages was by two-way ANOVA, using Šídák multiple comparison test, with *p < 0.05, **p < 0.01, ***p < 0.001. (A) Quantification of M2 polarisation represented as a ratio of CD206:CD68. (B) Quantification of M1 polarisation represented as a ratio of MHC Class II:CD68. (C) Representative images of cross sections at the proximal end of HS silk vascular grafts, white dotted lines indicate the graft wall. Upper two rows: CD206 stained in orange, CD68 stained in green and nuclei stained in blue. Lower two rows: MHC Class II stained in purple, CD68 stained in green and nuclei stained in blue. Scale bar = 200 μm.