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. 2019 Nov 1;8(12):1330–1341. doi: 10.1002/sctm.19-0113

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© 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Retention of bone marrow mesenchymal stromal cells (BMSCs) after transplantation. (A): BMSC sheets contained fibronectin and attached on the kidney surface accordingly. Scale bar: 100 μm. (B, C): Bioluminescence imaging following BMSC‐sheet transplantation (black line) revealing superior retention of donor cells as compared with those injected (black dotted line). In the intravenous (IV) group, pulmonary embolism was observed (arrow). (D): In the BMSC‐sheet group, green fluorescent protein‐expressing BMSCs (shown in red color) were observed on the kidney surface until day 14, with no accompanying migration of the transplanted cells into the parenchyma (). Green is laminin and blue is Hoechst. Scale bar: 200 μm; *, p < .05.

Inline graphic — Retention of bone marrow mesenchymal stromal cells (BMSCs) after transplantation. (A): BMSC sheets contained fibronectin and attached on the kidney surface accordingly. Scale bar: 100 μm. (B, C): Bioluminescence imaging following BMSC‐sheet transplantation (black line) revealing superior retention of donor cells as compared with those injected (black dotted line). In the intravenous (IV) group, pulmonary embolism was observed (arrow). (D): In the BMSC‐sheet group, green fluorescent protein‐expressing BMSCs (shown in red color) were observed on the kidney surface until day 14, with no accompanying migration of the transplanted cells into the parenchyma (). Green is laminin and blue is Hoechst. Scale bar: 200 μm; *, p < .05.