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. 2019 Sep 4;8(12):1242–1248. doi: 10.1002/sctm.19-0159

Figure 1.

Figure 1

Epidermal growth factor (EGF) and EGF‐hydrogel promotes primary astrocyte proliferation. (A): Representative immunostaining staining of GFAP positive (green, primary astrocyte) cells counterstained with DAPI. Scale bar = 20 μm. (B): GFAP positive cells number at 3 days after treated with/without gelatin hydrogel (Gtn) or with/without EGF. Error bars represent SEM, n = 16. (C): Water‐soluble tetrazolium salts (WST) assay of primary astrocytes treated with/without Gtn or with/without EGF at 3 days. Error bars represent SEM, n = 4. (D): qPCR analysis of astrocytes GFAP level at 3 days after treated with/without Gtn or with/without EGF. Data were normalized to its respective housekeeping gene (Hprt‐1) and control gene expression. Error bars represent SEM, n = 3; *, p < .05; **, p < .01; ***, p < .001.