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. 2019 Sep 4;8(12):1242–1248. doi: 10.1002/sctm.19-0159

Figure 2.

Figure 2

Astrocyte conditioned media (ACM) from astrocytes treated with epidermal growth factor (EGF)‐hydrogel reversed oxygen–glucose deprivation (OGD) induced neuronal death. (A): Schematic depiction of ACM transfer experiment. (B): Representative immunostaining staining of MAP‐2 positive (red, primary neurons) cells counterstained with DAPI. Scale bar = 20 μm. (C): MAP‐2 positive cells number at 24 hours after 2 hours OGD insult and replaced with neuron conditioned medium (−ve ACM, Gtn and EGF; control) or ACM cultured with/without gelatin hydrogel (Gtn) or EGF under normoxia condition. Error bars represent SEM, n = 12; ***, p < .001.