Table 1.
List of antibodies used for immunohistochemistry and flow cytometry
| Target | Vendor | Cat. N. | Application | Dilution | Incubation |
|---|---|---|---|---|---|
| E‐cadherin | Abcam | ab45990 | IHC | 1/200 | 1 hour, RT |
| PTF1A | Donated by Dr. Mastracci | n/a | IHC | 1/500 | 4°C, ON |
| SOX9 | Abnova | H00006662‐M02 | IHC | 1/100 | 2 hours, RT |
| PDX1 | Abcam | ab47383 | IHC | 1/500 | 1 hour, RT |
| FGFR2 | Abcam | ab10648 | IHC | 1/500 | 1 hour, RT |
| PCNA | Abcam | ab2426 | IHC | 1/1,000 | 1 hour, RT |
| Glucagon | Abcam | ab8055 | IHC | 1/100 | 1 hour, RT |
| Amylase | Donated by Dr. Mastracci | n/a | IHC | 1/500 | 4°C, ON |
| CPA1 | Novus Biologicals | NBP1‐47704 | IHC | 1/500 | 1 hour, RT |
| SOX9 | Abcam | ab196450 | FC | 1/50 × 107 cells | 30 minutes, ice |
| PTF1a | BD Pharmingen | 564750 | FC | 1/20 × 107 cells | 30 minutes, ice |
| FGFR2 | Novus Biologicals | FAB6843G | FC | 1/10 × 106 cells | 1 hour, ice |
| Mouse IgG1 | R&D systems | MAB002 | FC | 1/25 × 106 cells | 1 hour, ice |