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. 2019 Oct 21;8(12):1306–1317. doi: 10.1002/sctm.19-0145

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© 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Sirius red‐polarization/fluorescence detection of collagen fibers and immunohistochemical analysis of subdermical implants in rats. Pictures show analysis of sections obtained from decalcified implants (n = 4). (A): Polarization microscopy of Sirius red stained collagen fibers, showing birefringence of collagen enriched areas. (B): Slides observed under green fluorescent showing the typical autofluorescence of collagen fibers. (C): Immunohistochemistry analysis of the correspondent sections using a Col1a1 antibody where arrows indicate regions rich in COL1a1. Magnification: ×4.